COVID19_AstraZenecaVaccine
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SARS-CoV-2 candidate vaccine ChAdOx1 nCoV-19
"Background: ChAdOx1 nCoV-19 is a recombinant adenovirus vaccine candidate against SARS-CoV-2. Although replication defective in normal cells, 28kbp of adenovirus genes are delivered to the cell nucleus alongside the SARS-CoV-2 S glycoprotein gene.
Methods: We used direct RNA sequencing to analyse transcript expression from the ChAdOx1 nCoV-19 genome in human MRC-5 and A549 cell lines that are non-permissive for vector replication alongside the replication permissive cell line, HEK293. In addition, we used quantitative proteomics to study over time the proteome and phosphoproteome of A549 and MRC5 cells infected with the ChAdOx1 nCoV-19 vaccine candidate." https://www.researchsquare.com/article/rs-94837/v1
What does this mean?
"Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation and expression of genes."
"Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species" (Wikipedia)
"MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted male fetus." (Wikipedia)
"HEK293 = Human embryonic kidney 293 cells... are a specific cell line originally derived from human embryonic kidney cells grown in tissue culturetaken from an aborted female fetus. HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy." (Wikipedia)
"A549 cells are adenocarcinomic* human alveolar basal epithelial cells, and constitute a cell line that was first developed in 1972 by D. J. Giard, et al. through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old caucasian male. The cells are used as models for the study of lung cancer and the development of drug therapies against it." (Wikipedia)
*"Adenocarcinoma is a type of cancerous tumor that can occur in several parts of the body." (Wikipedia)
"Background: ChAdOx1 nCoV-19 is a recombinant adenovirus vaccine candidate against SARS-CoV-2. Although replication defective in normal cells, 28kbp of adenovirus genes are delivered to the cell nucleus alongside the SARS-CoV-2 S glycoprotein gene.
Methods: We used direct RNA sequencing to analyse transcript expression from the ChAdOx1 nCoV-19 genome in human MRC-5 and A549 cell lines that are non-permissive for vector replication alongside the replication permissive cell line, HEK293. In addition, we used quantitative proteomics to study over time the proteome and phosphoproteome of A549 and MRC5 cells infected with the ChAdOx1 nCoV-19 vaccine candidate." https://www.researchsquare.com/article/rs-94837/v1
What does this mean?
"Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation and expression of genes."
"Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species" (Wikipedia)
"MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted male fetus." (Wikipedia)
"HEK293 = Human embryonic kidney 293 cells... are a specific cell line originally derived from human embryonic kidney cells grown in tissue culturetaken from an aborted female fetus. HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy." (Wikipedia)
"A549 cells are adenocarcinomic* human alveolar basal epithelial cells, and constitute a cell line that was first developed in 1972 by D. J. Giard, et al. through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old caucasian male. The cells are used as models for the study of lung cancer and the development of drug therapies against it." (Wikipedia)
*"Adenocarcinoma is a type of cancerous tumor that can occur in several parts of the body." (Wikipedia)